2D97
Structure of VIL-xylanase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-08-01 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.347, 38.585, 57.162 |
Unit cell angles | 90.00, 110.31, 90.00 |
Refinement procedure
Resolution | 18.980 - 2.010 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.22800 |
Structure solution method | MIRAS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.980 | 2.130 |
High resolution limit [Å] | 2.010 | 2.010 |
Number of reflections | 11242 | |
Completeness [%] | 99.2 | 88.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 293 | 1.5M ammonium sulfate, 0.1M Na citrate, pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |