2D8W
Structure of HYPER-VIL-trypsin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-07-12 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.323, 58.197, 66.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.310 - 2.000 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.25600 |
Structure solution method | SIRAS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.811 | 2.130 |
High resolution limit [Å] | 1.999 | 2.000 |
Number of reflections | 14772 | |
Completeness [%] | 99.6 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2.0M ammonium sulfate, 60mM benzamidine, 6mM CaCl2, 50mM Tris/HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |