2D8N

Crystal structure of human recoverin at 2.2 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-03
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9790, 0.9794, 0.964
Spacegroup name	I 4
Unit cell lengths	85.034, 85.034, 65.689
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.520 - 2.200
R-factor	0.189
Rwork	0.189
R-free	0.23700
Structure solution method	MAD
RMSD bond length	0.020
RMSD bond angle	1.800
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.200	2.200
Number of reflections	11968
<I/σ(I)>	23.4162	9.35583
Completeness [%]	99.9	99.8
Redundancy	7.27097

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M HEPES-Na pH7.5, 1.2M tri-Sodium Citrate dihydrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K