2D80
Crystal structure of PHB depolymerase from Penicillium funiculosum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2004-11-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.507, 68.876, 38.733 |
| Unit cell angles | 90.00, 107.86, 90.00 |
Refinement procedure
| Resolution | 38.890 - 1.700 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.20400 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.072 | 0.360 |
| Number of reflections | 27251 | |
| <I/σ(I)> | 28.4 | 4.64 |
| Completeness [%] | 99.9 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 20% PEG 8000, 50mM potassium dihydrogenphosphate, 5% glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
