2D7T
Crystal structure of human anti polyhydroxybutyrate antibody Fv
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 63.409, 73.620, 103.299 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.1911 |
| Rwork | 0.190 |
| R-free | 0.21408 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ad9 1dee |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.608 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.046 | 0.142 |
| Number of reflections | 26775 | |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 99.4 | 99.3 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.3 | 293 | 20% PEG8000, 0.1M CHES, pH 9.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
