2D5R
Crystal Structure of a Tob-hCaf1 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-03 |
| Detector | OXFORD PX210 |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 151.505, 151.505, 114.181 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.270 - 2.500 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.24600 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.270 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 23262 | |
| Completeness [%] | 80.9 | 83.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 12% PEG4000, 0.08M Tris, 0.16M sodium acetate, 0.01M dithiothreitol, 12% glycerol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
