2D5M
Flavoredoxin of Desulfovibrio vulgaris (Miyazaki F)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.71000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 53.346, 53.346, 116.224 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.050 |
| R-factor | 0.1352 |
| Rwork | 0.128 |
| R-free | 0.16150 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.031 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.090 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Number of reflections | 89930 | |
| Completeness [%] | 99.6 | 97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 283 | PEG8K, Zn acetate, MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
