2D5H
Crystal Structure of Recombinant Soybean Proglycinin A3B4 subunit, its Comparison with Mature Glycinin A3B4 subunit, Responsible for Hexamer Assembly
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-10-16 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.378, 223.738, 88.660 |
| Unit cell angles | 90.00, 119.85, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.32500 |
| Structure solution method | MIR |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 2.870 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Number of reflections | 68145 | |
| Completeness [%] | 89.5 | 40.2 |
| Redundancy | 3.7 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 20% MPD, 0.1M MES pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
