2D4W
Crystal structure of glycerol kinase from Cellulomonas sp. NT3060
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-05 |
Detector | MARRESEARCH |
Wavelength(s) | 1.130 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 105.430, 105.430, 195.715 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.19535 |
Rwork | 0.193 |
R-free | 0.24046 |
Structure solution method | SIROAS |
RMSD bond length | 0.015 |
RMSD bond angle | 1.593 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.900 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.104 | |
Number of reflections | 49935 | |
<I/σ(I)> | 5.8 | |
Completeness [%] | 99.2 | 99.2 |
Redundancy | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.5 | 277 | 27-37% MPD, 0.1M citrate, pH 4.5, VAPOR DIFFUSION, temperature 277K |