2D48
Crystal structure of the Interleukin-4 variant T13D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2002-08-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9178 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 90.528, 90.528, 45.907 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.980 - 1.650 |
| R-factor | 0.22222 |
| Rwork | 0.221 |
| R-free | 0.24709 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hik |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.695 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.600 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.092 | 0.172 |
| Number of reflections | 22700 | |
| <I/σ(I)> | 17.6 | 5.2 |
| Completeness [%] | 97.1 | 98.1 |
| Redundancy | 6.7 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 52% Ammonium sulfate, 0.1M Sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
