2D3V

Crystal Structure of Leukocyte Ig-like Receptor A5 (LILRA5/LIR9/ILT11)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97921, 0.97956, 0.90000, 1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	28.080, 58.645, 131.619
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.850
R-factor	0.23547
Rwork	0.234
R-free	0.26861
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.551
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.052	0.382
Number of reflections	19328
<I/σ(I)>	16.6	2.5
Completeness [%]	92.1	49.2
Redundancy	6.3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	Bicine, PEG20000, Dioxane, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K