2D3T
Fatty Acid beta-oxidation multienzyme complex from Pseudomonas Fragi, Form V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL24XU |
| Synchrotron site | SPring-8 |
| Beamline | BL24XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-11 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 0.834 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.227, 137.552, 198.139 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.400 |
| R-factor | 0.248 |
| Rwork | 0.242 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wdk |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.823 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1000.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 35837 | |
| Completeness [%] | 97.9 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEG4000, MES, N-OCTYLPENTAOXYETHYLENE, NAD+, ACETOACETYL-COA, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
