2D3S

Crystal Structure of basic winged bean lectin with Tn-antigen

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2004-05-04
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	157.866, 91.897, 73.503
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.350
R-factor	0.204
Rwork	0.198
R-free	0.24400
Starting model (for MR)	PDB CODE 1wbl
RMSD bond length	0.006
RMSD bond angle	1.460
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.086	0.416
Number of reflections	44561
<I/σ(I)>	18.8	4.1
Completeness [%]	97.5	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	6-7% PEG4000,15% Isopropanol, 0.02M PBS, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K