2D3I
Crystal Structure of Aluminum-Bound Ovotransferrin at 2.15 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-07 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.353, 59.184, 87.974 |
| Unit cell angles | 90.00, 95.72, 90.00 |
Refinement procedure
| Resolution | 49.030 - 2.150 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ovt |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 40021 | |
| <I/σ(I)> | 16.5 | 4.2 |
| Completeness [%] | 98.4 | 94 |
| Redundancy | 5.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | PEG400, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
