2D32
Crystal Structure of Michaelis Complex of gamma-Glutamylcysteine Synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-14 |
| Detector | RIGAKU |
| Wavelength(s) | 0.9000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 325.530, 325.530, 105.003 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.400 |
| R-factor | 0.16994 |
| Rwork | 0.168 |
| R-free | 0.19844 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v4g |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.381 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.600 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.088 | 0.374 |
| Number of reflections | 162223 | |
| <I/σ(I)> | 6.1 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.1 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | sodium formate, Tris-HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
