2D2S

Crystal Structure of the Exo84p C-terminal Domains

Experimental procedure

Experimental method	MAD
Spacegroup name	P 43 21 2
Unit cell lengths	54.850, 54.850, 209.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.850
R-factor	0.273
Rwork	0.269
R-free	0.30600
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.950
High resolution limit [Å]	2.850	2.850
Number of reflections	13025
Completeness [%]	97.5	57.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	PEG 3000, iso-propanol, sodium cloride, dithiothreitol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K