2D2R
Crystal structure of Helicobacter pylori Undecaprenyl Pyrophosphate Synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL17B2 |
Synchrotron site | NSRRC |
Beamline | BL17B2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-02-11 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.630, 58.914, 153.425 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.880 |
Rwork | 0.193 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1x06 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.817 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.950 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.055 | 0.433 |
Number of reflections | 35917 | |
<I/σ(I)> | 30.7 | 4.1 |
Completeness [%] | 95.6 | 90.3 |
Redundancy | 5.6 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | KSCN, PEG600, PEG5KMME, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |