2D1O
Stromelysin-1 (MMP-3) complexed to a hydroxamic acid inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08-20 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.360, 50.950, 80.790 |
| Unit cell angles | 90.00, 105.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.020 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2srt |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.610 | 2.120 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Number of reflections | 26846 | |
| Completeness [%] | 96.7 | 79.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG400, MPD, hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
