2D1N

Collagenase-3 (MMP-13) complexed to a hydroxamic acid inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-02-11
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 32
Unit cell lengths	96.470, 96.470, 67.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	9.980 - 2.370
R-factor	0.218
Rwork	0.218
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	830c
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.370
Number of reflections	27785
Completeness [%]	97.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	potassium sodium tartrate, hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K