2D0W
Crystal structure of cytochrome cL from Hyphomicrobium denitrificans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-22 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.7 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.779, 57.283, 67.007 |
| Unit cell angles | 90.00, 101.55, 90.00 |
Refinement procedure
| Resolution | 29.240 - 1.980 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.240 | 2.070 |
| High resolution limit [Å] | 1.980 | 2.000 |
| Number of reflections | 22502 | |
| Completeness [%] | 95.0 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 289 | PEG10000, zinc sulfate, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
