2D0V
Crystal structure of methanol dehydrogenase from Hyphomicrobium denitrificans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-19 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 291.320, 63.999, 109.941 |
| Unit cell angles | 90.00, 105.74, 90.00 |
Refinement procedure
| Resolution | 44.600 - 2.490 |
| R-factor | 0.15854 |
| Rwork | 0.149 |
| R-free | 0.24687 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.328 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.600 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.500 |
| Number of reflections | 68299 | |
| Completeness [%] | 99.6 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | PEG3350, potassium thiocyanate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
