2D0J
Crystal Structure of Human GlcAT-S Apo Form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.978 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 110.402, 122.591, 92.985 |
Unit cell angles | 90.00, 108.20, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.000 |
R-factor | 0.2395 |
Rwork | 0.237 |
R-free | 0.27750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v82 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.149 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.049 | 0.258 |
Number of reflections | 76289 | |
<I/σ(I)> | 23.7 | 6.9 |
Completeness [%] | 96.0 | 90.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | PEG5000 MME, di-sodium hydrogen phosphate, cesium chloride, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |