2D0I
Crystal Structure PH0520 protein from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-21 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.756, 63.739, 131.978 |
| Unit cell angles | 90.00, 103.14, 90.00 |
Refinement procedure
| Resolution | 34.690 - 1.950 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.26500 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.089 | |
| Number of reflections | 101628 | |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 97.0 | 98.9 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.8 | 295 | Sodium Cacodylate, PEG 4000, pH 5.8, microbatch, temperature 295.0K |
| 1 | MICROBATCH | 5.8 | 295 | Sodium Cacodylate, PEG 4000, pH 5.8, microbatch, temperature 295.0K |
| 1 | MICROBATCH | 5.8 | 295 | Sodium Cacodylate, PEG 4000, pH 5.8, microbatch, temperature 295.0K |
