2CZV

Crystal structure of archeal RNase P protein ph1481p in complex with ph1877p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	63.332, 76.118, 151.276
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.690 - 2.000
R-factor	0.218
Rwork	0.218
R-free	0.26100
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.100
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.130
High resolution limit [Å]	2.000	2.000
Number of reflections	49669
Completeness [%]	99.0	96.8
Redundancy	6.3	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG 3350, magnesium acetate, n-octly b-glucoside, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG 3350, magnesium acetate, n-octly b-glucoside, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K