2CZ9
Crystal Structure of galactokinase from Pyrococcus horikoshi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-05-17 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 0.95 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 106.081, 38.905, 81.566 |
Unit cell angles | 90.00, 109.81, 90.00 |
Refinement procedure
Resolution | 28.140 - 1.500 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s4e |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.260 |
High resolution limit [Å] | 1.240 | 1.240 |
Rmerge | 0.054 | 0.464 |
Number of reflections | 86789 | |
<I/σ(I)> | 18.9 | 2.1 |
Completeness [%] | 97.4 | 94.2 |
Redundancy | 2.8 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.3 | 291 | PEG4000, magnesium chloride, MES, pH 6.3, Micro batch, temperature 291K |