2CYX

Structure of human ubiquitin-conjugating enzyme E2 G2 (UBE2G2/UBC7)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-17
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	63.522, 87.610, 157.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.430 - 2.560
Rwork	0.228
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ucz
RMSD bond length	0.009
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.650
High resolution limit [Å]	2.560	2.560
Number of reflections	28705
<I/σ(I)>	22.2	4.2
Completeness [%]	97.5	84.6
Redundancy	4.11	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.1	293	12% Glycerol, 0.1M Tris, 1.45M Ammonium Sulfate, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K