2CY9
Crystal structure of thioesterase superfamily member2 from Mus musculus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-20 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.97922, 0.97957, 0.964 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 66.522, 90.195, 61.210 |
Unit cell angles | 90.00, 118.32, 90.00 |
Refinement procedure
Resolution | 49.100 - 2.720 |
R-factor | 0.19404 |
Rwork | 0.191 |
R-free | 0.26243 |
Structure solution method | MAD |
RMSD bond length | 0.078 |
RMSD bond angle | 4.788 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 9667 | |
<I/σ(I)> | 8.58 | 2.9 |
Completeness [%] | 71.0 | 23.8 |
Redundancy | 2.79 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M Tris-HCl, 2.0 M ammonium sulphate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |