2CXL
RUN domain of Rap2 interacting protein x, crystallized in I422 space group
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-16 |
Wavelength(s) | 0.97888, 0.97951, 0.97600, 0.98300 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 112.774, 112.774, 76.476 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.100 - 3.200 |
R-factor | 0.28 |
Rwork | 0.280 |
R-free | 0.36000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1WUS |
RMSD bond length | 0.003 |
RMSD bond angle | 0.800 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.290 |
High resolution limit [Å] | 3.180 | 3.180 |
Number of reflections | 4293 | |
<I/σ(I)> | 15.2776 | 5.51505 |
Completeness [%] | 99.9 | 100 |
Redundancy | 10.0547 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 7.6 | 293 | PEG4000, Lithium Sulfate, Tris-HCl, pH 7.6, LIQUID DIFFUSION, temperature 293K |