2CXI
Crystal Structure Of An N-terminal Fragment Of The Phenylalanyl-tRNA Synthetase Beta-Subunit From Pyrococcus Horikoshii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97928, 0.97960, 0.98400 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.779, 81.973, 139.096 |
| Unit cell angles | 90.00, 116.79, 90.00 |
Refinement procedure
| Resolution | 41.520 - 1.940 |
| R-factor | 0.18981 |
| Rwork | 0.188 |
| R-free | 0.23070 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.546 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 104810 | |
| Completeness [%] | 99.4 | 94.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch, modified | 5.6 | 293 | PEG 20000, sodium citrate, pH 5.6, Microbatch, modified, temperature 293.0K |
