2CX9
Crystal structure of acyl-CoA dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-30 |
| Detector | RIGAKU |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.685, 88.637, 119.557 |
| Unit cell angles | 90.00, 91.15, 90.00 |
Refinement procedure
| Resolution | 49.550 - 2.000 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 104174 | |
| <I/σ(I)> | 16.1 | |
| Completeness [%] | 97.7 | 85.4 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 16.5% PEG20000, 0.1M HEPES, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
