2CX4

Crystal structure of a bacterioferritin comigratory protein peroxiredoxin from the Aeropyrum pernix K1 (form-2 crystal)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-06
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.96400, 0.97884, 0.97946
Spacegroup name	P 41
Unit cell lengths	132.479, 132.479, 106.588
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.320 - 2.300
R-factor	0.203
Rwork	0.203
R-free	0.26300
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	143980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	285	ammonium sulfate, MES, Tris, sodium chloride, dithiothreitol, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 285K