2CWT
Catalytic base deletion in copper amine oxidase from arthrobacter globiformis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 157.668, 63.840, 184.453 |
Unit cell angles | 90.00, 112.40, 90.00 |
Refinement procedure
Resolution | 30.015 - 1.820 |
R-factor | 0.191 |
Rwork | 0.191 |
R-free | 0.20450 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.950 |
High resolution limit [Å] | 1.800 | 1.850 |
Rmerge | 0.058 | |
Number of reflections | 144511 | |
Completeness [%] | 99.8 | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 6.8 | 289 | 1.05M POTASSIUM TARTRATE, 25mM HEPES, pH 6.8, MICRODIALYSIS, temperature 289K |