2CV4
Crystal Structure of an Archaeal Peroxiredoxin from the Aerobic Hyperthermophilic Crenarchaeon Aeropyrum pernix K1
Replaces: 1VGSExperimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-01 |
| Detector | RIGAKU |
| Wavelength(s) | 0.97933, 0.97000, 0.97905 |
| Spacegroup name | P 1 |
| Unit cell lengths | 76.738, 103.434, 102.814 |
| Unit cell angles | 105.18, 92.91, 105.39 |
Refinement procedure
| Resolution | 49.200 - 2.300 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.23000 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 120086 | |
| Completeness [%] | 84.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | oil microbatch method | 8 | 291 | ammonium sulfate, MES, dioxane, sodium chloride, DTT, Tris, pH 8, oil microbatch method, temperature 291K |
