2CSD
Crystal structure of Topoisomerase V (61 kDa fragment)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.500, 86.400, 175.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.800 - 2.900 |
R-factor | 0.23973 |
Rwork | 0.236 |
R-free | 0.30787 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.111 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.800 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 25958 | |
<I/σ(I)> | 6.7 | 2.3 |
Completeness [%] | 99.2 | 98.5 |
Redundancy | 3.7 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 295 | sodium acetate, sodium formate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 295K |