2CQZ
Crystal Structure of PH0347 protein from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-07 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.511, 101.166, 78.646 |
| Unit cell angles | 90.00, 119.78, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.600 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.22531 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xx7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.542 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.057 | |
| Number of reflections | 32607 | |
| <I/σ(I)> | 9 | |
| Completeness [%] | 99.7 | 98.8 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 9 | 295 | Sodium chloride, Bicine, PEG-MME 550, pH 9.0, microbatch, temperature 295K |
