2CO5
F93 FROM STIV, a winged-helix DNA-binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-17 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 42.050, 102.594, 92.398 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.370 - 2.200 |
R-factor | 0.192 |
Rwork | 0.190 |
R-free | 0.23200 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.007 |
RMSD bond angle | 0.991 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.060 | 0.340 |
Number of reflections | 10466 | |
<I/σ(I)> | 33 | 4.2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.1 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.75 | HANGING DROP VAPOR DIFFUSION, 9.0-10.5 MG/ML F93 IN 10 MM TRIS-HCL, PH 8.0, 50 MM NACL, 0.5 MM TCEP MIXED WITH BUFFER CONTAINING 0.1 M SODIUM ACETATE, PH 4.75-5.0, 0.1 M MAGNESIUM NITRATE HEXAHYDRATE, 16-20% PEG 20,000. |