2CN7
Recombinant human H ferritin, K86Q, E27D and E107D mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-18 |
| Detector | MARRESEARCH |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.207, 181.207, 181.207 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 104.830 - 1.750 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2clu |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.296 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.300 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.560 |
| Number of reflections | 26251 | |
| <I/σ(I)> | 26.5 | 5.34 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.7 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | HANGING DROP. RESERVOIR: MPD 5.2%, CACL2 3.9 MM, TRIS 50MM PH 8.5. DROP: 1UL PROTEIN (24 MG/ML) AND 1 UL RESERVOIR |
