2CN6
Recombinant human H ferritin, K86Q and E107D mutant, soaked with Zn ions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-18 |
Detector | MARRESEARCH |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 178.453, 178.453, 178.453 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 103.140 - 2.200 |
R-factor | 0.196 |
Rwork | 0.195 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB 2CHI |
RMSD bond length | 0.017 |
RMSD bond angle | 1.413 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.610 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.090 | 0.550 |
Number of reflections | 12895 | |
<I/σ(I)> | 28 | 7 |
Completeness [%] | 99.8 | 100 |
Redundancy | 23 | 23 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | HANGING DROP. RESERVOIR: MPD 5% CACL2 4.7 MM, TRIS 50 MM PH 8.5 DROP: 1 UL PROTEIN (22 MG/ML) AND 1 UL RESERVOIR |