2CN1
Crystal structure of Human Cytosolic 5'-Nucleotidase III (NT5C3)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-25 |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 86.389, 100.951, 76.723 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.650 - 2.670 |
R-factor | 0.196 |
Rwork | 0.192 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bdu |
RMSD bond length | 0.015 |
RMSD bond angle | 1.610 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.700 | 2.810 |
High resolution limit [Å] | 2.670 | 2.670 |
Rmerge | 0.090 | 0.360 |
Number of reflections | 9828 | |
<I/σ(I)> | 15.2 | 4.2 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 5.5 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 24% PEG1500, 20% GLYCEROL, pH 7.50 |