2CMK
CYTIDINE MONOPHOSPHATE KINASE IN COMPLEX WITH CYTIDINE-DI-PHOSPHATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 291 |
Detector technology | IMAGE PLATE |
Collection date | 1996-10-07 |
Detector | MARRESEARCH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 77.268, 77.268, 81.232 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.000 - 2.000 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NON LIGATED CMPK |
RMSD bond length | 0.012 |
RMSD bond angle | 23.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.84) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.050 * | 0.425 * |
Total number of observations | 286435 * | |
Number of reflections | 17252 | |
<I/σ(I)> | 25.7 | 3 |
Completeness [%] | 98.4 | 95.9 |
Redundancy | 16.6 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.4 | pH 7.4 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | CDP | 20 (mM) | |
3 | 1 | drop | ammonium sulfate | 0.4 (M) | |
4 | 1 | reservoir | ammonium sulfate | 1.5 (M) |