2CM2
Structure of Protein Tyrosine Phosphatase 1B (P212121)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS-IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.640, 69.210, 100.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| Rwork | 0.198 |
| R-free | 0.23350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eeo |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.538 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNX |
| Refinement software | CNX (2000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.320 |
| Number of reflections | 44327 | |
| <I/σ(I)> | 16 | 3 |
| Completeness [%] | 96.0 | 73 |
| Redundancy | 4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.9 | 100 MM SODIUM/POTASSIUM PHOSPHATE PH 5.9 AND 25%-35% METHYLPENTANEDIOL |
