2CLD

CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT FORM OF H-RAS P21 IN COMPLEX WITH GDP (2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-01
Detector	ADSC CCD
Spacegroup name	P 41
Unit cell lengths	69.410, 69.410, 34.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.340 - 1.220
R-factor	0.15
Rwork	0.149
R-free	0.17600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4q21
RMSD bond length	0.011
RMSD bond angle	1.534
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.340	1.250
High resolution limit [Å]	1.220	1.220
Rmerge	0.050	0.460
Number of reflections	47013
<I/σ(I)>	21.5	3
Completeness [%]	94.1	55.5
Redundancy	6.09	4.21

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	291.15	PROTEIN SOLUTION: 13.35 MG/ML PROTEIN, 64 MM TRIS PH 7.6, 20 MM MAGNESIUM CHLORIDE, 10 MM DTT, 0,1 MM SODIUM AZIDE; RESERVOIR SOLUTION: 100 MM HEPES PH 7.2, 200 MM MAGNESIUM ACETATE, 16% PEG 8000 (FRESHLY PREPARED) MIXTURE OF EQUAL VOLUMES OF PROTEIN AND RESERVOIR SOLUTION, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291.15K