2CKY
Structure of the Arabidopsis thaliana thiamine pyrophosphate riboswitch with its regulatory ligand
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.850, 98.670, 54.550 |
| Unit cell angles | 90.00, 104.80, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.900 |
| R-factor | 0.191 |
| Rwork | 0.183 |
| R-free | 0.25000 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.218 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.235 | 0.970 |
| Number of reflections | 8918 | |
| <I/σ(I)> | 7.6 | 1.6 |
| Completeness [%] | 96.0 | 74 |
| Redundancy | 3.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10% 1,6-HEXANEDIOL 50MM SODIUM CACODYLATE PH7.0 10MM MGSO4 |
