2CKX
Crystal structure of NgTRF1, double-stranded telomeric repeat binding factor from Nicotiana tabacum.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 290 |
| Detector technology | CCD |
| Collection date | 2006-01-26 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.122, 48.265, 52.006 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.2101 |
| Rwork | 0.210 |
| R-free | 0.23270 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.218 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.800 | 1.900 |
| Rmerge | 0.040 | 0.060 |
| Number of reflections | 9807 | |
| Completeness [%] | 98.2 | 96.9 |
| Redundancy | 2.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1M TRIS PH 8.5, 25%(W/V) PEG3350 |
