2CJQ
Bovine viral diarrhea virus CP7-R12 RNA-dependent RNA polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 31 |
| Unit cell lengths | 106.244, 106.244, 55.387 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.2311 |
| Rwork | 0.231 |
| R-free | 0.25870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s48 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.437 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.120 | 0.380 |
| Number of reflections | 21373 | |
| <I/σ(I)> | 14.3 | 2.5 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 5.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 10% POLYETHYLENE GLYCOL 8000, 0.1M HEPES PH 7.5, 8% ETHYLENE GLYCOL |
