2CIU
Structure of the IMS domain of the mitochondrial import protein Tim21 from S. cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-29 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.310, 58.820, 62.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.1975 |
| Rwork | 0.198 |
| R-free | 0.24830 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.680 |
| High resolution limit [Å] | 1.600 | 1.580 |
| Rmerge | 0.060 | 0.330 |
| Number of reflections | 16515 | |
| <I/σ(I)> | 18 | 3.72 |
| Completeness [%] | 97.5 | 94 |
| Redundancy | 3.33 | 3.27 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.2 M LI2SO4, 0.1 M BIS-TRIS PH 6.5, 25% PEG 3350 |
