2CI1
Crystal Structure of dimethylarginine dimethylaminohydrolase I in complex with S-nitroso-Lhomocysteine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-17 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.664, 79.910, 44.894 |
Unit cell angles | 90.00, 108.58, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.080 |
R-free | 0.14130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h70 |
RMSD bond length | 0.015 |
RMSD bond angle | 0.034 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.120 |
High resolution limit [Å] | 1.080 | 1.080 |
Rmerge | 0.080 | 0.340 |
Number of reflections | 114012 | |
<I/σ(I)> | 15.7 | 4.1 |
Completeness [%] | 98.6 | 96 |
Redundancy | 3.65 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 100 MM CITRIC ACID/NAOH, 20-40% PEG 8000, 2 MM TCEP, PH 5.0 |