2CHL
Structure of casein kinase 1 gamma 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-25 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 55.802, 55.802, 219.707 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.950 |
| R-factor | 0.186 |
| Rwork | 0.183 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c47 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.597 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.510 | 2.050 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.100 | 0.510 |
| Number of reflections | 29588 | |
| <I/σ(I)> | 14.49 | 1.7 |
| Completeness [%] | 98.2 | 88.5 |
| Redundancy | 8.7 | 3.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.0 M LI2SO4, 0.5 M TMAO |
