2CE8
An EH1 peptide bound to the Groucho-TLE WD40 domain.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-09-02 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 107.585, 56.352, 125.245 |
Unit cell angles | 90.00, 112.32, 90.00 |
Refinement procedure
Resolution | 116.250 - 2.030 |
R-factor | 0.182 |
Rwork | 0.179 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gxr |
RMSD bond length | 0.018 |
RMSD bond angle | 1.804 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.120 |
High resolution limit [Å] | 2.020 | 2.020 |
Rmerge | 0.060 | 0.190 |
Number of reflections | 87651 | |
<I/σ(I)> | 13.5 | 5.2 |
Completeness [%] | 95.0 | 74.5 |
Redundancy | 2.9 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.5 | 287 | 6MG/ML PROTEIN WAS MIXED 1:1 WITH 12% PEG8000 100MM NACACODYLATE, 100MM CAACETATE AT 14C IN MICROBATCH. PEPTIDES WERE ADDED TO THE PROTEIN CRYSTALS IN 125 MM NACL, 25 MM TRIS PH8.0, 0.5 MM EDTA TO A FINAL CONCENTRATION OF 0.76 MM, AND INCUBATED FOR 16 H BEFORE HARVESTING AND WASHING IN 50MM NA CACODYLATE PH6.5, 12 % PEG 8K, AND CRYO-PROTECTED IN 30% ETHYLENE GLYCOL 50MM NA CACODYLATE PH6.5, 12 % PEG 8K, pH 6.50 |