2CDZ

CRYSTAL STRUCTURE OF THE HUMAN P21-ACTIVATED KINASE 4 IN COMPLEX WITH CGP74514A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-11-17
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 43 21 2
Unit cell lengths	145.817, 145.817, 42.382
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	103.140 - 2.300
R-factor	0.202
Rwork	0.200
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bva
RMSD bond length	0.012
RMSD bond angle	1.430
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.070	0.410
Number of reflections	23590
<I/σ(I)>	12.9	4
Completeness [%]	98.7	92.2
Redundancy	6.76	5.67

Crystallization Conditions

crystal ID	method	pH	temperature	details
1